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Catalog Number | orb767279 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to HDAC6 (phospho-Ser22) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC6 around the phosphorylation site of Ser22. AA range:7-56 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 131 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-HDAC6 antibody, anti-KIAA0901 antibody, anti- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, HDAC6 (phospho Ser22) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 Monoclonal Antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Western Blot analysis of 3T3 cells using Phospho-HDAC6 (S22) Polyclonal Antibody diluted at 1:500.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC6 (Phospho-Ser22) Antibody.
ICC, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
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