HDAC5 (Phospho-S259/220) antibody

• Catalog Number: orb393071
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HDAC5 (Phospho-S259/220)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rabbit
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S259/220 of human Histone Deacetylase 5/9 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HDAC5; HDAC9
• Entrez: 79221, 10014, 9734
• UniProt ID: Q9UKV0, Q9Z2V6, Q99N13, Q9UQL6
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone Deacetylase 5/9 (Phospho-S259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 121; 111 kD; Observed band size: 124 kD)

Immunohistochemical analysis of Histone Deacetylase 5/9 (Phospho-S259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.