HDAC5 Antibody

• Catalog Number: orb546306
• Category: Antibodies
• Description: HDAC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HDAC5 recombinant protein (Position: H102-E197).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 150 kDa
• UniProt ID: Q9UQL6
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC5
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of HDAC5 using anti-HDAC5 antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human THP-1 cell;4:human K562 cell;5:human PC-3 cell;6:human Caco-2 cell.

WB analysis of HDAC5 using anti-HDAC5 antibody.Lane 1:rat heart tissue;2:rat liver tissue;3:rat stomach tissue;4:rat ovary tissue;5:mouse heart tissue;6:mouse liver tissue;7:mouse stomach tissue;8:mouse ovary tissue;9:mouse NIH3T3 cell.

IHC analysis of HDAC5 using anti-HDAC5 antibody.HDAC5 was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of HDAC5 using anti-HDAC5 antibody.HDAC5 was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of HDAC5 using anti-HDAC5 antibody.HDAC5 was detected in paraffin-embedded section of rat small intestine tissue.