HDAC4 (Phospho-Ser246) antibody

• Catalog Number: orb157455
• Category: Tools
• Description: HDAC4 (Phospho-Ser246) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246 (TA(p-S)EP)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 119 kDa
• Target: Phospho-HDAC4 (Ser246)
• UniProt ID: P56524
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: HDAC4(Phospho-Ser246); HDAC4(Phospho-S246); p-HDAC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Hela(Human) Cell Lysate at 30 ug. 293T(Human) Cell Lysate at 30 ug. Primary: Anti- Phospho-HDAC4 (Ser246) (orb157455) at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 119 kD. Observed ban

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30