HCG-beta Antibody

• Catalog Number: orb385662
• Category: Antibodies
• Description: This antibody reacts with a protein of 22kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90% and 60% of cases respectively; 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Purified HCG-beta protein was used as the immunogen for this antibody.
• Tested applications: IHC-Fr
• Dilution range: Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Clone HCGb/211 (recommended capture) will pair with clones HCGb/459 and HCGb/54 (recommended detect) by ELISA.
• Antibody Type: Primary Antibody
• Clone Number: HCGb/211
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This HCG-beta antibody is available for research use only.
• Note: For research use only

SDS-PAGE Analysis of Purified, BSA-Free HCG-beta Antibody (clone HCGb/211). Confirmation of Integrity and Purity of the Antibody.