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HCCS Antibody (Center)
Catalog Number: orb1927668
| Catalog Number | orb1927668 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB24043 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:4000, IHC-P: 1:50~100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 30602 Da |
| Target | This HCCS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-200 amino acids from the Central region of human HCCS. |
| UniProt ID | P53701 |
| NCBI | NP_005324.3, NP_001116080.1, NP_001165462.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Cytochrome c-type heme lyase, CCHL, Holocytochrome Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-HCCS Antibody (Center) at 1:4000 dilution. Lane 1: human heart lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
HCCS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCCS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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