HBG2 Antibody

• Catalog Number: orb1265011
• Category: Antibodies
• Description: HBG2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This HBG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-133 amino acids from the C-terminal region of human HBG2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 16 kDa
• Target: HBG2
• UniProt ID: P69892
• NCBI: P69892
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F G
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (green) compared to an isotype control of rabbit IgG (blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from K562 cell line and human placenta tissue lysate (from left to right), using HBG2 Antibody. was diluted at 1:1000 at each lane.