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Catalog Number | orb1520362 |
---|---|
Category | Antibodies |
Description | HAUS5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 75 and 125 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | HAUS5 |
UniProt ID | O94927 |
NCBI | O94927.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | dgt5; HAUS augmin-like complex subunit 5; KIAA0841 Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human HAUS5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HAUS5 antibody (orb1520362) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human HAUS5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HAUS5 antibody (orb1520362) used for IP at 1 µg per reaction. For blotting immunoprecipitated HAUS5, orb1520362 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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