HAO1 Mouse Monoclonal Antibody

• Catalog Number: orb500001
• Category: Antibodies
• Description: HAO1 Mouse Monoclonal Antibody
• Target: HAO1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: Recombinant mouse HAO1 Protein full length
• UniProt ID: Q9WU19
• MW: 41 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 6G8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Glycolate oxidase; GOX; GOX1; HAO1; HAOX1; HAOX1_H
• Research Area: Cell Biology, Energy Metabolism, Fatty Acid Oxidat
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HAO1) Monoclonal Antibody, Unconjugated (ascites of orb500001 Mix) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-HAO1 (orb500001) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 42 kD.