HAO1 Mouse Monoclonal Antibody

SKU: orb500001

Description

HAO1 Mouse Monoclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 6G8
• Immunogen: Recombinant mouse HAO1 Protein full length
• Target: HAO1
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GO; GOX; GOX1; HAOX1; Hao-1; HAOX1_HUMAN; HAO1; Glycolate oxidase (GO | GOX); Glyoxylate oxidase; 1.1.3.15; HAOX1_MOUSE; Glycolate oxidase (GOX);

Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HAO1) Monoclonal Antibody, Unconjugated (ascites of orb500001 Mix) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-HAO1 (orb500001) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 42 kD.

Quick Database Links

Gene Symbol

HAO1

UniProt

Q9WU19