HAND2 Antibody

• Catalog Number: orb1267177
• Category: Antibodies
• Description: HAND2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Gallus, Mouse, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human HAND2.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: HAND2
• UniProt ID: P61296
• NCBI: P61296
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Heart- and neural crest derivatives-expressed prot
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of Hela cell stained with HAND2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HAND2 immunoreactivity is localized to Nucleus significantly.

Western blot analysis in K562 cell line lysates (35 ug/lane).