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    Hakai Antibody

    Catalog Number: orb1525681

    DispatchUsually dispatched within 5-10 working days
    $ 626.00
    Catalog Numberorb1525681
    CategoryAntibodies
    DescriptionHakai Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIP, WB
    ReactivityHuman, Mouse
    Immunogenbetween 441 and 491
    Concentration1000 µg/ml
    Dilution rangeWB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
    Form/AppearanceWhole IgG
    ConjugationUnconjugated
    TargetHakai
    UniProt IDQ75N03
    NCBINP_079090.2
    Storage2 - 8°C
    Buffer/PreservativesTris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
    Alternative namesRING-type E3 ubiquitin transferase Hakai; RNF188;
    Read more...
    NoteFor research use only
    Application notesFormat: Whole IgG
    Expiration Date12 months from date of receipt.
    Hakai Antibody

    Detection of human and mouse Hakai by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Hakai antibody orb1525681 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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      Polyclonal

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