Haemoglobin Antibody [2A1], Rabbit IgG

• Catalog Number: orb1671813
• Category: Antibodies
• Description: Haemoglobin Antibody [2A1], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 2A1
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: Murine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend’s Adjuvant.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: P01942
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Hemoglobin, Hb
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-Haemoglobin antibody 2A1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric r version of 2A1 (orb1671813) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671813- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.