H4R3me2a Antibody

• Catalog Number: orb1258172
• Category: Antibodies
• Description: H4R3me2a Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 13kDa
• Target: H4R3me2a
• UniProt ID: P62805
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H4-R3 antibody (orb1258172). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (orb1258172).

Immunohistochemistry of paraffin-embedded rat brain using Asymmetric DiMethyl-Histone H4-R3 antibody (orb1258172) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using Asymmetric DiMethyl-Histone H4-R3 antibody (orb1258172) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H4-R3 antibody (orb1258172) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H4-R3 antibody (orb1258172). Blue: DAPI for nuclear staining.