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Catalog Number | orb1257934 |
---|---|
Category | Antibodies |
Description | H3R2me2a Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, IF, IHC, IP, WB |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 17kDa |
Target | H3R2me2a |
UniProt ID | Q16695 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | H3F3A, H3t, H3.4, H3/g, H3FT Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody (orb1257934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody (orb1257934).
Immunohistochemistry of paraffin-embedded rat brain using H3R2me2a antibody (orb1257934) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using H3R2me2a antibody (orb1257934) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H3R2me2a antibody (orb1257934) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R2 antibody (orb1257934). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R2 antibody (orb1257934) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Asymmetric DiMethyl-Histone H3-R2 antibody (orb1257934) at dilution of 1:100. Blue: DAPI for nuclear staining.
ChIP, ICC, IF, IP, WB | |
All, Human, Mouse, Rat | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IP, WB | |
Human, Mouse, Other, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
DOT, IF, IHC, WB | |
C. elegans, Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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