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Catalog Number | orb1257936 |
---|---|
Category | Antibodies |
Description | H3R26me2s Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 17kDa |
Target | H3R26me2s |
UniProt ID | P68431 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936).
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Symmetric DiMethyl-Histone H3-R26 antibody (orb1257936) at dilution of 1:100. Blue: DAPI for nuclear staining.
ELISA, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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