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Catalog Number | orb1258180 |
---|---|
Category | Antibodies |
Description | H3K79me2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Other, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human DiMethyl-Histone H3-K79 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 17kDa |
Target | H3K79me2 |
UniProt ID | Q16695 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | H3F3A, H3t, H3.4, H3/g, H3FT Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K79 antibody (orb1258180). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (orb1258180).
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (orb1258180) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (orb1258180) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (orb1258180) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (orb1258180). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (orb1258180) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ChIP, ICC, IF, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IHC, IP, WB | |
Human, Mouse, Other, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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