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Catalog Number | orb1258191 |
---|---|
Category | Antibodies |
Description | H3K4me3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Other, Rat |
Isotype | IgG |
Immunogen | A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 18kDa |
Target | H3K4me3 |
UniProt ID | Q16695 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | H3F3A, H3t, H3.4, H3/g, H3FT Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K4 antibody (orb1258191). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (orb1258191) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K4 antibody (orb1258191). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody (orb1258191) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
IHC-P | |
Bovine, Drosophila, Human, Mouse, Porcine, Rabbit, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ChIP, ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ICC, IF, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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