H3K36me3 Antibody

• Catalog Number: orb1258182
• Category: Antibodies
• Description: H3K36me3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Other, Rat
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 18kDa
• Target: H3K36me3
• UniProt ID: Q16695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K36 antibody (orb1258182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K36 antibody (orb1258182).

Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K36 antibody (orb1258182) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K36 antibody (orb1258182) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K36 antibody (orb1258182) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K36 antibody (orb1258182). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K36 antibody (orb1258182) and rabbit IgG. P1 and P2 were located on GAPDH gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.