H3K14me1 Antibody

• Catalog Number: orb1257585
• Category: Antibodies
• Description: H3K14me1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human MonoMethyl-Histone H3-K14
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: H3K14me1
• UniProt ID: Q16695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K14 antibody (orb1257585). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H3-K14 pAb (orb1257585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K14 pAb (orb1257585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.