H1F0 Antibody

• Catalog Number: orb1274222
• Category: Antibodies
• Description: H1F0 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HH1/1784R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H1F0
• UniProt ID: P07305
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: H1F0, H10, H1FV, MGC5241
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.