GTF2I Antibody / TFII-I

• Catalog Number: orb1823001
• Category: Antibodies
• Description: Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-GTF2I-1A7
• Tested applications: FACS
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant human protein was used as the immunogen for the GTF2I antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GTF2I antibody is available for research use only.
• UniProt ID: P78347
• Storage: Aliquot the GTF2I antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using GTF2I antibody (clone PCRP-GTF2I-1A7). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Flow cytometry testing of PFA-fixed human HeLa cells with GTF2I antibody (clone PCRP-GTF2I-1A7) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free GTF2I antibody (clone PCRP-GTF2I-1A7) as confirmation of integrity and purity.