GTF2I Antibody (C-term)

• Catalog Number: orb1788469
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 956-985 aa
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 112416 Da
• Target: This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I.
• UniProt ID: P78347
• NCBI: NP_001267729.1, NP_127492.1, NP_127494.1, NP_001157108.1, NP_127493.1, NP_001509.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GTF2I; BAP135; WBSCR6; General transcription facto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Hela, A431 cell line (from left to right), using GTF2I Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with GTF2I Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-GTF2I Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: A431 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 112 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.