GTF2H1 monoclonal antibody (M01), clone 1F12-1B5

• Catalog Number: orb2292856
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant GTF2H1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1F12-1B5
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: GTF2H1 (AAH00365, 1 a.a. ~ 548 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT
• NCBI: AAH00365
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged GTF2H1 is approximately 1 ng/ml as a capture antibody.

GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Western Blot analysis of GTF2H1 expression in Jurkat.

GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Western Blot analysis of GTF2H1 expression in Hela S3 NE.

Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue. [antibody concentration 5 ug/ml]

Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Lane 1: GTF2H1 transfected lysate (62 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # orb2292856). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (86.02 KDa).