GTF2E1/TFIIE-alpha Antibody

• Catalog Number: orb1526052
• Category: Antibodies
• Description: GTF2E1/TFIIE-alpha Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 100 and 150
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-GTF2E1/TFIIE-alpha antibody A302-755A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GTF2E1/TFIIE-alpha
• UniProt ID: P29083
• NCBI: NP_005504.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: TFIIE alpha subunit; TFIIE-alpha; TFIIE-A; transcr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human GTF2E1/TFIIE-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody orb1526052 used for IP at 3 µg/mg lysate.

Detection of human GTF2E1/TFIIE-alpha by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha (Cat. No. orb1526052) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB