GST Antibody

SKU: orb413639

Description

Mouse monoclonal antibody to GST

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB (1:1000); ELISA (1:800)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SMC-549 was sufficient for detection of GST in 1 µg of Purified GST tagged PmtA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 300
• Immunogen: Glutathione-S-Transferase (GST) tagged protein
• Target: GST
• Molecular Weight: 26 kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Glutathione S Transferase, Glutathione S-transferase class-mu 26 kDa isozyme, GST, Sj26 antigen, SjGST, Glutathione-S-Transferase, GST tag, GST tagged protein, GST fusion protein, GST protein

Western Blot analysis of Schistosoma japonicum Purified GST showing detection of 26 kDa GST protein using Mouse Anti-GST Monoclonal Antibody, Clone 3E2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein. Lane 3: GST. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GST Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 26 kDa.

Quick Database Links

Gene Symbol

GST

UniProt

P08515