You have no items in your shopping cart.
Cart summary
| Catalog Number | orb1946755 |
|---|---|
| Category | Assays and Kits |
| Description | GSSG(Oxidized Glutathione) ELISA Kit |
| Target | GSSG |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Oxidized Glutathione(GSSG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oxidized Glutathione(GSSG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oxidized Glutathione(GSSG) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Time | 1.5H |
| Range | 312.5-20000 ng/mL |
| Sensitivity | 91.3 ng/mL |
| Sample Types | biological agents and other biological fluids |
| Alternative names | Oxidized Glutathione |
| Note | For research use only |
