GSPT1 Antibody (N-term)

• Catalog Number: orb1925266
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB24635
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55756 Da
• Target: This GSPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-29 amino acids from the N-terminal region of human GSPT1.
• UniProt ID: P15170
• NCBI: NP_001123479.1, NP_001123478.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Eukaryotic peptide chain release factor GTP-bindin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

GSPT1 Antibody (N-term) western blot analysis in U251, CEM cell line lysates (35 ug/lane). This demonstrates the GSPT1 antibody detected the GSPT1 protein (arrow).

GSPT1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

GSPT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSPT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.