GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody

• Catalog Number: orb2318863
• Category: Antibodies
• Description: GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: SPM249
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Isotype: IgG2a
• Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 94kDa
• Target: HSP90B1
• Entrez: 7184
• UniProt ID: P14625
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Glucose Regulated Protein 94; 94kDa glucose-regula
• Note: For research use only
• Application notes: Positive Control: HePG2. U20S cells. About 50% of breast tumors express grp94 protein
• Expiration Date: 12 months from date of receipt.