GRLF1/p190RhoGAP Antibody

• Catalog Number: orb1527883
• Category: Antibodies
• Description: GRLF1/p190RhoGAP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1200 and 1250
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GRLF1/p190RhoGAP
• UniProt ID: Q9NRY4
• NCBI: EAW57452.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: rho GTPase-activating protein 35; rho GAP p190A; P
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse GRLF1/p190RhoGAP by western blot. Samples: Whole cell lysate (50 µg) from CT26.WT, 4T1, and Renca cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody orb1527883 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GRLF1/p190RhoGAP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody orb1527883 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. GRLF1/p190RhoGAP was also immunoprecipitated by rabbit anti-GRLF1/p190RhoGAP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).