GRIK5 Antibody

• Catalog Number: orb1249536
• Category: Antibodies
• Description: GRIK5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-GPAGPRELAEHE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx. 26kDa band in Human Brain (Frontal Cortex and Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we can not find an explanation in the literature for the band we observe given the calculated size of 109kDa according to NP_002079.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GRIK5
• UniProt ID: Q16478
• NCBI: NP_002079.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GRIK5, glutamate receptor, ionotropic, kainate 5,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx. 26kDa band in Human Brain (Frontal Cortex and Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we can not find an explanation in the literature for the band we observe given the calculated size of 109kDa according to NP_002079.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249536 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.