GRIA4 antibody

• Catalog Number: orb20006
• Category: Antibodies
• Description: Goat polyclonal antibody to GRIA4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 5-10µg/ml
• Conjugation: Unconjugated
• MW: 101; 99.2; 49.1
• Target: GRIA4
• Entrez: 2893
• Protein Sequence: KKLDQREYPGSETP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GRIA4 antibody, anti glutamate receptor, iono
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10ug/ml.WB: Approx. 90 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kDa according to NP_001070711.1). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20006 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20006 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.