GPX4 antibody

• Catalog Number: orb19310
• Category: Antibodies
• Description: Goat polyclonal antibody to GPX4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 0.3 μg/ml
• Conjugation: Unconjugated
• MW: 22.1; 27.1
• Target: GPX4 (Isoform a and c)
• Entrez: 2879
• Protein Sequence: EEPLVIEKDLPHY
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GPX4 antibody, anti glutathione peroxidase 4
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3ug/ml.WB: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19310 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19310 (0.3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.