GPRC6A antibody

• Catalog Number: orb515453
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPRC6A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPRC6A. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GPRC6A
• Entrez: 222545, 294394, 210198
• UniProt ID: Q8K4Z6, Q5T6X5, Q70VB1
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: G-protein coupled receptor family C group 6 member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPRC6A expression in HEK293T (A), LO2 (B), rat testis (C) whole cell lysates. (Predicted band size: 104 kD; Observed band size: 120 kD)

Immunofluorescent analysis of GPRC6A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.