GPRC5D antibody

• Catalog Number: orb515232
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPRC5D
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPRC5D. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GPRC5D
• Entrez: 55507, 93746
• UniProt ID: Q9NZD1, Q9JIL6
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: G-protein coupled receptor family C group 5 member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPRC5D expression in MCF7 (A), SGC7901 (B), Panc1 (C), CT26 (D), PC12 (E) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 39 kD)

Immunofluorescent analysis of GPRC5D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.