GPRC5B antibody

• Catalog Number: orb215188
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPRC5B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPRC5B. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GPRC5B
• Entrez: 51704, 64297
• UniProt ID: Q923Z0, Q9NZH0
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti RAIG2 antibody, anti G-protein coupled recept
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPRC5B expression in A375 (A), DLD (B), mouse testis (C), rat liver (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 45 kD)

Immunofluorescent analysis of GPRC5B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.