GPR176 antibody

• Catalog Number: orb215050
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPR176
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR176. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GPR176
• Entrez: 381413, 11245, 117257
• UniProt ID: Q14439, Q64017, Q80WT4
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Probable G-protein coupled receptor 176 antib
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPR176 expression in HEK293T (A), Hela (B), U2OS (C), mouse testis (D), mouse heart (E), rat testis (F), rat heart (G) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)

Immunofluorescent analysis of GPR176 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.