You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1289810 |
---|---|
Category | Antibodies |
Description | Rabbit Polyclonal antibody to GPR146 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR146. The exact sequence is proprietary. |
Dilution range | WB: 1:500-1:1000, IF:IC: 1:50-1:200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | GPR146 |
Entrez | 115330 |
UniProt ID | Q96CH1 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | PGR8; Probable G-protein coupled receptor 146; G-p Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of GPR146 expression in A549 (A), Hela (B), rat lung (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)
Immunofluorescent analysis of GPR146 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Filter by Rating