GPR125 Antibody

• Catalog Number: orb1246542
• Category: Antibodies
• Description: GPR125 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: CTNKSASSFKNS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml. A customer reported successful results on Human Testis.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GPR125
• UniProt ID: Q8IWK6
• NCBI: NP_660333.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GPR125 , G protein-coupled receptor 125 , PGR21, T
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml. A customer reported successful results on Human Testis.
• Expiration Date: 12 months from date of receipt.

orb1246542 (0.5 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246542 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.