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GPNMB Rabbit Polyclonal Antibody

Catalog Number: orb6109

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6109
CategoryAntibodies
DescriptionGPNMB Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Guinea pig, Porcine
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GPNMB (81-180/572aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW61 kDa
TargetGPNMB
UniProt IDQ14956
RRIDAB_10932687
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesNMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL;
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NoteFor research use only
Expiration Date12 months from date of receipt.
GPNMB Rabbit Polyclonal Antibody

Sample: Lane 1: Recombinant human GPNMB protein, C-His (HEK293), Primary: Anti-GPNMB (orb6109) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 65 kDa.

GPNMB Rabbit Polyclonal Antibody

Tissue/Cell: rat lung tissue (melanoma), 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (orb6109) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • GPNMB Rabbit Polyclonal Antibody [orb579191]

    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • GPNMB Rabbit Polyclonal Antibody [orb579193]

    WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • GPNMB Rabbit Polyclonal Antibody [orb579192]

    WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • GPNMB Rabbit Polyclonal Antibody (PE) [orb485180]

    IF

    Bovine, Equine, Guinea pig, Porcine

    Human, Rat

    Rabbit

    Polyclonal

    PE

    100 μl
  • Anti-GPNMB Antibody [orb666816]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl