You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb6109 |
---|---|
Category | Antibodies |
Description | GPNMB Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Bovine, Equine, Guinea pig, Porcine |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human GPNMB (81-180/572aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 61 kDa |
Target | GPNMB |
UniProt ID | Q14956 |
RRID | AB_10932687 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | NMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Sample: Lane 1: Recombinant human GPNMB protein, C-His (HEK293), Primary: Anti-GPNMB (orb6109) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 65 kDa.
Tissue/Cell: rat lung tissue (melanoma), 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (orb6109) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF | |
Bovine, Equine, Guinea pig, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
PE |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |