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Catalog Number | orb2291147 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant GOLM1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3B10 |
Tested applications | ELISA, IP, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | GOLM1 (NP_057632, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | VQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL |
NCBI | NP_057632 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged GOLM1 is 0.1 ng/ml as a capture antibody.
Immunoprecipitation of GOLM1 transfected lysate using anti-GOLM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GOLM1 MaxPab rabbit polyclonal antibody.
Western Blot analysis of GOLM1 expression in transfected 293T cell line by GOLPH2 monoclonal antibody (M04), clone 3B10. Lane 1: GOLM1 transfected lysate(45.3 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.74 KDa).