Golgin subfamily A member 4 antibody

• Catalog Number: orb411691
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Golgin subfamily A member 4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Golgin-245
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GOLGA4
• Entrez: 2803, 54214
• UniProt ID: Q5U4E6, Q91VW5, Q13439
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-256 kDa golgin antibody, anti-72.1 protein an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Golgin-245 expression in Hela (A), A549 (B), mouse heart (C), mouse brain (D) whole cell lysates. (Predicted band size: 260; 261; 262 kD; Observed band size: 280 kD)

Immunofluorescent analysis of Golgin-245 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).