Golgi Antibody / GLG1

• Catalog Number: orb1716285
• Category: Antibodies
• Description: This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GLG1/4830
• Tested applications: IHC
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the GLG1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (Frozen & FFPE): 0.5-1ug/ml (No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues)
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GLG1 antibody is available for research use only.
• UniProt ID: Q92896
• Storage: Aliquot the GLG1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the GLG1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with GLG1 antibody (clone GLG1/4830).