GOLGA6B Antibody

• Catalog Number: orb1247434
• Category: Antibodies
• Description: GOLGA6B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DLPKEKADGTEQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 45kDa band in human liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.9kDa according to NP_001033729.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows localized cytoplasm staining consistent with Golgi. Recommended concentration, 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GOLGA6B
• UniProt ID: A6NDN3
• NCBI: NP_061122.4, NP_001033729.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GOLGA6B, GOLGA6, golgi autoantigen, golgin subfami
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 45kDa band in human liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.9kDa according to NP_001033729.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows localized cytoplasm staining consistent with Golgi. Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247434 (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.