Goat Ig Human IgD Immunofixation antibody

• Catalog Number: orb21783
• Category: Antibodies
• Description: Ig fraction of polyclonal goat antiserum to human immunoglobulin IgD, Fc specific. This antiserum is intended to detect and identify IgD in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
• Species/Host: Goat
• Clonality: Polyclonal
• Form/Appearance: Goat Ig lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Source: Highly purified monoclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Storage: Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product
• Buffer/Preservatives: Goat Ig lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
• Note: For research use only
• Application notes: Immunoprecipitation.
• Expiration Date: 12 months from date of receipt.