Goat HeatShock Protein 70 (HSP-70/HSPA9) ELISA Kit

SKU: orb1146821

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HeatShock Protein 70(HSP-70/HSPA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HeatShock Protein 70(HSP-70/HSPA9) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Goat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.35 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

DnaK, 70 Kilodalton Heat Shock Proteins, Heat shock 70 kDa protein 1, 2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1, HSP70 2, HSP70 1A, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B, HSP70, Heat Shock Protein 70