SV2A Antibody

SKU: orb19294

Description

Goat polyclonal antibody to SV2A

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 80kDa band observed in rat brain lysates (calculated MW of 82.6kDa according to NP_476558.1 (rat)). Recommended concentration: 0.3-1 μg/ml. An additonal band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SV2A
• Protein Sequence: TVELYPSDKRTTA
• Molecular Weight: 82.7; 20.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SV2A antibody, anti synaptic vesicle glycoprotein 2A antibody, anti KIAA0736 antibody, anti SV2 antibody, anti synaptic vesicle glycoprotein 2 antibody

Western blot analysis of Rat Brain (A) + peptide (B) lysate using SV2A antibody.

Quick Database Links

Gene Symbol

SV2A

NCBI Gene ID

9900