SATB1 Antibody

SKU: orb20363

Description

Goat polyclonal antibody to SATB1

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  IHC: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration, 3-5μg/ml.WB: Experiments gave bands at approx. 110kDa and 85kDa in lysates of cell line MOLT4 after 0.3 μg/ml antibody staining. The observed 110kDa band corresponds to earlier findings in literature with different antibodies (De Belle et al, J Cell Biol. 1998 Apr 20;141(2):335-48.; PMID: 9548713). This protein has a calculated MW of 86.0kDa according to NP_002962.1. Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SATB1
• Protein Sequence: ESDEENRQKTRPRT
• Molecular Weight: 86.0. 89.0; 78.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SATB1 antibody, anti SATB homeobox 1 antibody, anti DNA-binding protein SATB1 antibody, anti special AT-rich sequence binding protein 1 antibody, anti special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA\1s) antibody

Western blot analysis of MOLT4 lysate (35µg protein in RIPA buffer) using SATB1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human thymus tissue using SATB1 antibody

Quick Database Links

Gene Symbol

SATB1

NCBI Gene ID

6304