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Item 1 of 4
Item 1 of 4
Goat anti-Pericentrin 1 / NUP85 Antibody
Catalog Number: orb18851
| Catalog Number | orb18851 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to NUP85 |
| Target | Pericentrin 1 / NUP85 |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | ARAIIREGSLEGS |
| RRID | AB_10748786 |
| Tested applications | ELISA, FC, IF, WB |
| Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml |
| Application notes | WB: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. |
| Alternative names | NUP85; pericentrin 1; PCNT1; PCN; PCNT; frount; nu Read more... |
| Note | For research use only |
0.5 μg/ml staining of HeLa (A), (0.3 μg/ml) NIH3T3 (B) and (1 μg/ml) nuclear HeLa (C) and nuclear HEK293 (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
