Pericentrin 1/NUP85 Antibody

SKU: orb18851

Description

Goat polyclonal antibody to NUP85

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  WB: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Key Properties

• Host: Goat
• Target: Pericentrin 1 / NUP85
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

NUP85; pericentrin 1; PCNT1; PCN; PCNT; frount; nucleoporin Nup75; nucleoporin 85kDa; FLJ12549; Nup75; nucleoporin 85

0.5 μg/ml staining of HeLa (A), (0.3 μg/ml) NIH3T3 (B) and (1 μg/ml) nuclear HeLa (C) and nuclear HEK293 (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

Pericentrin 1 / NUP85