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Goat anti-Pericentrin 1 / NUP85 Antibody

Catalog Number: orb18851

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18851
CategoryAntibodies
DescriptionGoat polyclonal antibody to NUP85
Species/HostGoat
Tested applicationsELISA, FC, IF, WB
ReactivityHuman, Mouse, Rat
Dilution rangeELISA: 1:32000, WB: 1-3 μg/ml
ConjugationUnconjugated
TargetPericentrin 1 / NUP85
Protein SequenceARAIIREGSLEGS
RRIDAB_10748786
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesNUP85; pericentrin 1; PCNT1; PCN; PCNT; frount; nu
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NoteFor research use only
Application notesWB: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
Expiration Date12 months from date of receipt.
Goat anti-Pericentrin 1 / NUP85 Antibody

0.5 μg/ml staining of HeLa (A), (0.3 μg/ml) NIH3T3 (B) and (1 μg/ml) nuclear HeLa (C) and nuclear HEK293 (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-Pericentrin 1 / NUP85 Antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Goat anti-Pericentrin 1 / NUP85 Antibody

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Goat anti-Pericentrin 1 / NUP85 Antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.