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Catalog Number | orb18851 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to NUP85 |
Species/Host | Goat |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
Target | Pericentrin 1 / NUP85 |
Protein Sequence | ARAIIREGSLEGS |
RRID | AB_10748786 |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | NUP85; pericentrin 1; PCNT1; PCN; PCNT; frount; nu Read more... |
Note | For research use only |
Application notes | WB: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. |
Expiration Date | 12 months from date of receipt. |
0.5 μg/ml staining of HeLa (A), (0.3 μg/ml) NIH3T3 (B) and (1 μg/ml) nuclear HeLa (C) and nuclear HEK293 (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.