Pallidin Antibody

SKU: orb18645

Description

Goat polyclonal to Pallidin

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 23-24kDa band observed in lysates of cell line A431 (calculated MW of 19.7kDa according to NP_036520.1). In transfected HEK293 transiently expressing PLDN a band of approx. 28kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Pallidin
• Protein Sequence: EREKQLTARPAKRM
• Molecular Weight: 19.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti pallidin homolog (mouse) antibody, anti pallidin antibody, anti PLDN antibody, anti PA antibody, anti PALLID antibody, anti pallid (mouse) homolog, pallidin antibody, anti syntaxin 13 binding protein 1 antibody, anti syntaxin 13-interacting protein pallid antibody

orb18645 staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing PLDN (RC201514) and probed with orb18645 (mock transfection in first lane), tested by Origene.

Quick Database Links

Gene Symbol

Pallidin

NCBI Gene ID

26258