Pallidin Antibody

• Catalog Number: orb18645
• Category: Antibodies
• Description: Goat polyclonal to Pallidin
• Target: Pallidin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EREKQLTARPAKRM
• RRID: AB_10755583
• MW: 19.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 23-24kDa band observed in lysates of cell line A431 (calculated MW of 19.7kDa according to NP_036520.1). In transfected HEK293 transiently expressing PLDN a band of approx. 28kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti pallidin homolog (mouse) antibody, anti palli
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 26258

orb18645 staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing PLDN (RC201514) and probed with orb18645 (mock transfection in first lane), tested by Origene.