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Catalog Number | orb18645 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to Pallidin |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 19.7 |
Target | Pallidin |
Entrez | 26258 |
Protein Sequence | EREKQLTARPAKRM |
RRID | AB_10755583 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti pallidin homolog (mouse) antibody, anti palli Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 23-24kDa band observed in lysates of cell line A431 (calculated MW of 19.7kDa according to NP_036520.1). In transfected HEK293 transiently expressing PLDN a band of approx. 28kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb18645 staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing PLDN (RC201514) and probed with orb18645 (mock transfection in first lane), tested by Origene.