Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody

• Catalog Number: orb2203722
• Category: Antibodies
• Description: Goat Anti-CRHR1 / CRF-R (aa 107 - 117) Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: CRHR1
• Dilution range: This antibody has been successfully used in ICC/IF on Rat: Zhong Xie, Peter Penzes, Deepak P. SrivastavaEPAC2 is required for corticotropin-releasing hormone-mediated spine lossApril 2019: doi: https://doi.org/10.1101/607598PMID: 0This antibody has been successfully used in Western blot on Mouse:Baglietto-Vargas D, Chen Y, Suh D, Ager RR, Rodriguez-Ortiz CJ, Medeiros R, Myczek K, Green KN, Baram TZ, LaFerla FM. Short-term modern life-like stress exacerbates A-beta-pathology and synapse loss in 3xTg-AD mice.J Neurochem. 2015 Sep;134(5):915-26.PMID: 26077803This antibody has been successfully used in Western blot on Mouse:Weerachayaphorn J, Mennone A, Soroka CJ, Harry K, Hagey LR, Kensler TW, Boyer JL.Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine.Am J Physiol Gastrointest Liver Physiol. 2012 May 1;302(9):G925-36.PMID: 22345550This antibody has been successfully used in IHC on Mouse: Jungling K, Liu X, Lesting J, Coulon P, Sosulina L, Reinscheid RK, Pape HC.Activation of neuropeptide S expressing neurons in the locus coeruleus by corticotropin-releasing factor.J Physiol. 2012 May 8.PMID: 22570383.
• Form/Appearance: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• MW: 47.7
• UniProt ID: P34998
• Protein Sequence: C-NEEKKSKVHYH
• NCBI: NP_004373.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: corticotrophin releasing hormone receptor 1;cortic
• Note: For research use only
• Application notes: Application Info: P-ELISA: Antibody detection limit dilution 1:128000WB: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3 ug/ml. Primary incubation was 1 hour.
• Expiration Date: 12 months from date of receipt.